Cobalt(III) as a Stable and Inert Mediator Ion between NTA and His6-Tagged Proteins**
نویسندگان
چکیده
Don't let go! The Co(3+)-mediated interaction between nitrilotriacetic acid (NTA) and the His6-tag is so stable and inert towards ligand exchange that it has a half-life of 7 days in the presence of imidazole and survives even under strongly chelating as well as reducing conditions, unlike the commonly used Ni(2+) or Co(2+) complexes. Possible applications include the separation of labeled proteins and the stable immobilization of proteins on surfaces.
منابع مشابه
Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on ni-nitrilotriacetic acid surfaces.
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عنوان ژورنال:
دوره 52 شماره
صفحات -
تاریخ انتشار 2013